| Solution Information | help | |
| Enzyme: | Orexin/Hypocretin receptor type 1 | |
| inhibitor: | BDBM79889 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose response curves for compounds identified as active in a previous set of experiments entitled, "FRET-based cell-based primary high throughput screening assay to identify antagonists of the orexin 1 receptor (OX1R; HCRTR1)" (AID 485270), and that confirmed activity in a previous set of experiments entitled, "Homogeneous Time Resolved Fluorescence (HTRF)-based cell-based high throughput confirmation assay for antagonists of the orexin 1 receptor (OX1R; HCRTR1)" (AID 492964). Compounds tested in this assay were inactive in a set of experiments entitled, "Counterscreen for antagonists of the orexin 1 receptor (OX1R; HCRTR1): Homogenous time resolved fluorescence (HTRF)-based cell-based assay to identify antagonists of the parental CHO-K1 cell line" (AID 493232). This assay employs the IP-One competitive immunoassay kit (Cisbio International) and CHO cells that stably express the Gq-coupled human receptor OX1R/Hcrtr1 (CHO-OX1R | |
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